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Related Experiment Video

Updated: May 25, 2026

Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma
06:15

Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma

Published on: October 27, 2014

Get back TFIIF, don't let me Gdown1.

Joaquín M Espinosa1

  • 1Howard Hughes Medical Institute, The University of Colorado, Boulder, CO 80309, USA. joaquin.espinosa@colorado.edu

Molecular Cell
|January 17, 2012
PubMed
Summary

The Gdown1 protein inhibits RNA polymerase II (RNAPII) transcription by antagonizing the TFIIF factor. The Mediator complex resolves this inhibition to enable RNAPII activation.

Area of Science:

  • Molecular biology
  • Gene transcription
  • Protein-protein interactions

Background:

  • The general transcription factor TFIIF is crucial for RNA polymerase II (RNAPII) transcription initiation and elongation.
  • The Gdown1 protein is known to interact with components of the transcription machinery.

Discussion:

  • This study elucidates the antagonistic role of Gdown1 against TFIIF during RNAPII transcription.
  • The Mediator complex acts as a critical regulator, modulating the Gdown1-TFIIF interaction.
  • This reveals a novel regulatory mechanism controlling RNAPII activity.

Key Insights:

  • Gdown1 directly antagonizes TFIIF, inhibiting RNAPII activity at initiation and elongation stages.
  • The Mediator complex intervenes to overcome Gdown1-mediated inhibition, facilitating RNAPII activation.

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Published on: September 2, 2015

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Last Updated: May 25, 2026

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06:15

Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma

Published on: October 27, 2014

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
10:30

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Published on: September 2, 2015

  • This molecular tug-of-war highlights intricate regulation of gene expression.

    • Outlook:

    • Further structural studies could detail the precise interaction interfaces between Gdown1, TFIIF, and Mediator.
    • Understanding this pathway may offer new targets for modulating gene transcription in disease contexts.