Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunological consequences of chemotherapy: implications for cancer immunotherapy.

Journal for immunotherapy of cancer·2026
Same author

Emerging roles and regulatory mechanisms involved in glutamine metabolism.

Trends in biochemical sciences·2026
Same author

Ras promotes macropinocytic nutrient uptake by suppressing the albumin recycling receptor FcRn.

EMBO reports·2026
Same author

Endogenous CD28 takes the driver's seat in 4-1BB co-stimulated CAR T cells.

Molecular therapy. Oncology·2026
Same author

Loss of p300/CBP-associated factor aggravates cardiac remodeling via regulation of CAMKK2 acetylation.

Experimental & molecular medicine·2026
Same author

Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 25, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

Ars2 promotes proper replication-dependent histone mRNA 3' end formation.

Joshua J Gruber1, Scott H Olejniczak, Jeongsik Yong

  • 1Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA.

Molecular Cell
|January 17, 2012
PubMed
Summary

Ars2 protein is crucial for histone mRNA processing and expression. Its interaction with 7SK RNA negatively regulates these functions, impacting cellular proliferation.

More Related Videos

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

Related Experiment Videos

Last Updated: May 25, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Processing

Background:

  • Ars2 (Arsenic resistance 2) is a key factor in the nuclear cap-binding complex, essential for microRNA biogenesis and cell proliferation.
  • Histone mRNAs possess a unique 3' end processing mechanism involving cleavage rather than polyadenylation, distinguishing them from most mammalian mRNAs.

Purpose of the Study:

  • To investigate the role of Ars2 in the regulation of histone mRNA processing and expression.
  • To identify the specific subset of mRNAs regulated by Ars2, with a focus on histone transcripts.
  • To elucidate the interplay between Ars2, histone mRNAs, and the noncoding RNA 7SK.

Main Methods:

  • Depletion of Ars2 using knockdown techniques.
  • Analysis of histone mRNA processing and polyadenylation status.
  • Co-immunoprecipitation assays to detect physical interactions between Ars2, histone mRNAs, and 7SK RNA.
  • Knockdown of 7SK RNA to assess its impact on histone mRNA processing in an Ars2-dependent manner.

Main Results:

  • Ars2 depletion led to a significant decrease in correctly processed histone mRNAs and a corresponding increase in polyadenylated histone transcripts.
  • Ars2 was found to physically associate with both histone mRNAs and 7SK RNA.
  • Knockdown of 7SK RNA enhanced the ratio of cleaved to polyadenylated histone transcripts, an effect contingent on the presence of Ars2.

Conclusions:

  • Ars2 plays a critical role in the 3' end formation and expression of histone mRNAs.
  • The functional activities of Ars2 in histone mRNA processing are negatively modulated by its interaction with 7SK RNA.
  • These findings reveal a novel regulatory mechanism for histone gene expression involving Ars2 and 7SK RNA.