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Updated: May 25, 2026
Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Determination of ganciclovir in human plasma by ultra performance liquid chromatography-UV detection
A Padullés1, H Colom, Y Armendariz
1Pharmacy Department, IDIBELL, Hospital Universitari de Bellvitge, L'hospitalet de Llobregat, Barcelona, Spain.
Objectives:
Implement a sensitive UHPLC method for the assay of ganciclovir in human plasma.
Design And Methods:
We developed and validated a chromatographic method coupled to ultraviolet detection for quantification of ganciclovir, with a short run time using a small volume of human plasma. Comparison of system performance was made with respect to analysis time, efficiency and sensitivity.
Results:
Correlation coefficients (r) of the calibration curves ranged from 0.999744 to 0.999784. Within-day and between-day imprecision and inaccuracy, specificity and recovery were also evaluated for validation. The method was precise and accurate and the retention time was 0.7 min. The calibration curves were linear between 0.5 and 30 μg/mL. There was a good correlation between HPLC and UHPLC techniques.
Conclusions:
We developed a method that is currently applied in a clinical study assessing GCV plasma concentration variability after ganciclovir and valganciclovir administration.
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