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Updated: May 25, 2026
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
Published on: May 12, 2018
Role of spinal cord in regulating mouse kidney: a virally mediated trans-synaptic tracing study
Da-Wei Ye1, Rong-Chun Li, Wei Wu
1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Objective:
To determine the spinal innervation and neuronal connections is important for studying renal metabolic responses.
Materials And Methods:
In this study, the spinal cords of 10 adult male C57BL/6J strain mice were mapped retrograde using injections of pseudorabies virus (PRV)-614. The virus, injected into the kidney, was specifically transported to the spinal cord.
Results:
At 5 days after injection of the PRV-614, PRV-614-positive cells were found in the intermediolateral cell column, the intercalates nucleus, or the central autonomic nucleus of spinal cord segments T4-L1, and most PRV-614-labeled cells were found in the T9 segment.
Conclusion:
Our results revealed neuroanatomical circuits between kidney and the spinal intermediolateral cell column neurons.
