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The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae
V L Souliotis1, M Patrinou-Georgoula, V Zongza
1National Hellenic Research Foundation, University of Athens, Greece.
Abstract:
The cuticle proteins of the insect Dacus oleae have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L3DCPs 1-7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis.