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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: May 25, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Affinity-based proteomic profiling: problems and achievements.

Alexei Medvedev1, Arthur Kopylov, Olga Buneeva

  • 1Institute of Biomedical Chemistry, Moscow, Russian Federation. professor57@yandex.ru.

Proteomics
|January 17, 2012
PubMed
Summary
This summary is machine-generated.

Affinity chromatography is a key technique in proteomics for protein separation and profiling. It aids in identifying drug targets and understanding drug side effects.

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Area of Science:

  • Biochemistry
  • Proteomics
  • Chromatography

Background:

  • Affinity chromatography is increasingly utilized in proteomic studies for protein subproteome separation.
  • This technique is crucial for analyzing diverse protein groups.

Purpose of the Study:

  • To review the application of affinity chromatography in proteomic profiling.
  • To highlight its role in characterizing small-molecule protein targets for drug discovery.

Main Methods:

  • Affinity chromatography for protein fractionation.
  • Proteomic analysis of specific protein groups.
  • Characterization of protein targets for small molecules.

Main Results:

  • Affinity chromatography enables profiling of cyclic nucleotide-binding proteins, protein kinases, phosphoproteins, glycoproteins, and ubiquitinated proteins.
  • It is vital for identifying direct protein targets of potential drug substances.
  • This method aids in evaluating drug side effects and potential applications.

Conclusions:

  • Affinity chromatography is an essential tool for advanced proteomic analysis.
  • Its application in drug discovery is critical for target identification and safety assessment.