In vivo rodent micronucleus assay of gmelina arborea roxb (gambhari) extract

Gmelina arborea Roxb (family Verbenaceae) commonly known as 'Gambhari' tree, the various parts of the plants are widely used in diarrhoea, anti-pyretic, thirst, anemia, leprosy, ulcers, consumption, strangury, vaginal discharges. We tested the genotoxic potential of G. arborea in bone marrow cells obtained from Swiss albino mice using micronuclei formation as the toxicological endpoints. Aqueous extract of G. arborea (AEGA) was tested at the dose of 286 & 667 mg/kg body weight (b. w.). Cyclophosphamide (CPZ) 25 mg/kg b. w. was used as positive control in micronucleus test. The AEGA significantly increased the % micronucleated polychrometics at doses of 286mg/kg and 667mg/kg, after 24, 48 72h time interval. And also decreased the PCE/NCE ratio after 24, 48 and 72 h as compared to solvent control group. In this study, we investigated the effect of G. arborea on mammalian bone marrow cells using micronuclei formation to assess the genotoxicity of the herb.