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Coupling of Na+ with the spermidine transporter protein in NIH BALB/c 3T3 cells
N A Khan1, I Masson, V Quemener
1Dept. of Cell Biology, Central Hospital University (C.H.U.) of Rennes I, Faculty of Medicine, France.
Cellular and Molecular Biology
|January 1, 1990
Abstract:
This study demonstrates that polyamine spermidine (Spd) transporter protein is directly coupled with the Na+ in a ternary complex form, Na(+)-Spd-carrier. The Spd is transported with Na+ in a 1:1 stoichiometry relationship. Interestingly, addition of 2-deoxyglucose in the assay medium did not influence significantly the Spd uptake demonstrating the ATP independency of Spd transport.