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Related Experiment Video

Updated: May 25, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
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A two-photon turn-on probe for glucose uptake.

Chang Su Lim1, Chul Chung, Hwan Myung Kim

  • 1Department of Chemistry, Korea University, 145-Anam-ro, Sungbuk-ku, Seoul, 136-713, Korea.

Chemical Communications (Cambridge, England)
|January 18, 2012
PubMed
Summary

Researchers developed a novel two-photon turn-on probe (AS1) for visualizing glucose uptake. This probe allows for real-time monitoring of intracellular glucose concentration changes in live cells and tissues.

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Area of Science:

  • Biomedical Optics
  • Molecular Imaging
  • Cell Biology

Background:

  • Accurate monitoring of glucose metabolism is crucial for understanding cellular function and disease.
  • Existing methods for glucose detection in live systems have limitations in resolution and real-time imaging.

Purpose of the Study:

  • To develop and validate a novel fluorescent probe for sensitive and specific detection of glucose.
  • To enable visualization of glucose uptake and intracellular concentration dynamics in live biological samples.

Main Methods:

  • Synthesis and characterization of a two-photon turn-on fluorescent probe (AS1).
  • Excitation of the probe using 780 nm femtosecond pulses.
  • Imaging of glucose uptake and concentration changes in live cells and tissue using two-photon microscopy.

Main Results:

  • The AS1 probe demonstrated efficient two-photon excitation at 780 nm.
  • The probe successfully visualized glucose uptake and dynamic changes in intracellular glucose concentration.
  • High-resolution imaging of glucose metabolism in live cellular and tissue models was achieved.

Conclusions:

  • The AS1 probe is a powerful tool for real-time, in vivo monitoring of glucose metabolism.
  • This technology has potential applications in diagnostics and understanding metabolic diseases.
  • Two-photon microscopy with AS1 offers a significant advancement in cellular and tissue-based glucose sensing.