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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

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Related Experiment Video

Updated: May 25, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Identification of abnormal stem cells using Raman spectroscopy.

Linda Harkness1, Sergey M Novikov, Jonas Beermann

  • 1Molecular Endocrinology Laboratory-KMEB, Odense University Hospital, Odense, Denmark.

Stem Cells and Development
|January 19, 2012
PubMed
Summary

Identifying abnormal stem cells for safe transplantation is crucial. Raman microscopy offers a rapid, label-free method to distinguish normal from abnormal stem cells by analyzing biomolecular composition, improving cell therapy safety.

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

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Last Updated: May 25, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Spectroscopy

Background:

  • Clinical application of stem cells for degenerative diseases necessitates robust methods for ensuring transplant safety.
  • Current methods for distinguishing normal from abnormal stem cells involve time-consuming in vivo and ex vivo testing.

Purpose of the Study:

  • To investigate Raman microscopy as a rapid, label-free technique for identifying abnormal stem cells.
  • To differentiate between normal and genetically abnormal/transformed stem cells using their unique spectral fingerprints.

Main Methods:

  • Raman spectroscopy was employed to map biomolecular distribution in adult human bone marrow-derived stromal stem cells and human embryonic stem cells.
  • Spectral characteristics of normal and abnormal stem cell populations were analyzed for reproducible differences.

Main Results:

  • Raman spectroscopy successfully mapped biomolecular composition in two distinct stem cell types.
  • Distinct and reproducible spectral differences were identified between normal and abnormal/transformed stem cells.
  • The technique demonstrated potential for label-free identification of abnormal stem cells.

Conclusions:

  • Raman microscopy provides a sensitive and rapid method for detecting abnormal stem cells.
  • This technique can be prospectively used for pre-clinical screening of stem cells in ex vivo cultures.
  • Raman microscopy enhances the safety and efficacy of stem cell-based therapeutics.