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Updated: May 25, 2026

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

Published on: December 8, 2023

Two new C19-diterpenoid alkaloids from Aconitum transsectum.

Yong Shen¹, Hong-Lian Ai, Shu-Hui Zi

¹School of Agriculture and Biological Technic, Yunnan Agricultural University, Kunming, 650201, China.

Journal of Asian Natural Products Research

|January 19, 2012

Summary

Two new C(19)-diterpenoid alkaloids, aconitramines D and E, were discovered in Aconitum transsectum roots. Their chemical structures were identified using advanced spectroscopic methods.

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2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

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Area of Science:

Natural Product Chemistry
Phytochemistry
Organic Chemistry

Background:

Aconitum species are known sources of bioactive alkaloids.
Diterpenoid alkaloids represent a significant class of natural products with diverse biological activities.
The chemical diversity within the Aconitum genus is extensive, necessitating further exploration.

Purpose of the Study:

To isolate and characterize novel C(19)-diterpenoid alkaloids from Aconitum transsectum.
To elucidate the chemical structures of the isolated compounds using comprehensive spectroscopic techniques.

Main Methods:

Isolation of alkaloids from the roots of Aconitum transsectum.
Structure elucidation utilizing Infrared (IR) spectroscopy.
Mass spectrometry (ESI-MS, HR-ESI-MS) for molecular weight determination.
Nuclear Magnetic Resonance (NMR) spectroscopy (1D and 2D) for detailed structural analysis.

Main Results:

Two new C(19)-diterpenoid alkaloids, designated aconitramines D (1) and E (2), were successfully isolated.
The structure of aconitramine D (1) was determined to be 18-demethoxyltransconitine A.
The structure of aconitramine E (2) was elucidated as 8-O-anisoyl-14-hydroxylacoforesticine.

Conclusions:

The study successfully identified and characterized two previously unknown diterpenoid alkaloids from Aconitum transsectum.
The findings contribute to the understanding of the phytochemical diversity of the Aconitum genus.
The elucidated structures provide a basis for future investigations into the biological activities of these novel compounds.