Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Scale-Up Processes

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

Designing Growth Media for Bioreactors

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Production of Antibiotics

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

Techniques for Isolation of Pure Cultures

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fast measurement of phosphates and ammonium in fermentation-like media: A feasibility study.

New biotechnology·2019

Same author

Quantitative Flow Cytometry to Understand Population Heterogeneity in Response to Changes in Substrate Availability in <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae</i> Chemostats.

Frontiers in bioengineering and biotechnology·2019

Same author

An integrated strategy for the effective production of bristle protein hydrolysate by the keratinolytic filamentous bacterium Amycolatopsis keratiniphila D2.

Waste management (New York, N.Y.)·2019

Same author

The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures.

Biotechnology progress·2019

Same author

CFD predicted pH gradients in lactic acid bacteria cultivations.

Biotechnology and bioengineering·2018

Same author

Exploring the potential of the glycerol-3-phosphate dehydrogenase 2 (GPD2) promoter for recombinant gene expression in <i>Saccharomyces cerevisiae</i>.

Biotechnology reports (Amsterdam, Netherlands)·2017

Search research articles

Related Experiment Video

Updated: May 25, 2026

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

Published on: July 29, 2025

Robust, small-scale cultivation platform for Streptomyces coelicolor.

Sujata Vijay Sohoni¹, Prashant Madhusudan Bapat, Anna Eliasson Lantz

¹Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Building 223, DK-2800 Kgs Lyngby, Denmark.

Microbial Cell Factories

|January 19, 2012

Summary

This study developed a microtiter plate (MTP) system for high-throughput screening of filamentous bacteria like Streptomyces coelicolor. The MTP platform accurately predicts bench-scale fermentation performance, improving reproducibility and reducing costs for secondary metabolite production.

More Related Videos

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Related Experiment Videos

Last Updated: May 25, 2026

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

Published on: July 29, 2025

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Area of Science:

Microbiology
Biotechnology
Fermentation Technology

Background:

High-throughput cultivation systems are essential for screening numerous conditions and strains in fermentation processes.
Existing microtiter plate (MTP) platforms are limited for filamentous organisms and lack systematic validation against larger scales.
Small-scale screening often provides only endpoint data, hindering dynamic process understanding.

Purpose of the Study:

To develop and validate a robust, small-scale microtiter plate (MTP) cultivation system for filamentous bacteria.
To compare the performance of the MTP platform with traditional shake flasks and bench-scale reactors.
To assess the scalability and reproducibility of physiological traits for secondary metabolite production.

Main Methods:

Developed a 24-square deepwell MTP cultivation system for Streptomyces coelicolor.
Optimized medium and inoculum preparation recipes for improved reproducibility.
Incorporated glass beads to enhance oxygen transfer and modify morphology.
Utilized MOPS buffer for pH control.

Main Results:

Redesigned MTPs with optimized conditions significantly reduced process time and improved reproducibility.
Glass beads enhanced oxygen transfer, secondary metabolite production, and shifted morphology from pellet to dispersed.
MTP cultivations showed good agreement with bench-scale reactors in growth rate, productivity, and substrate uptake.
Shake flask cultivations exhibited morphological discrepancies and lower antibiotic production rates.

Conclusions:

The developed MTP platform provides reliable physiological data comparable to bench-scale fermentation.
This MTP system is highly suitable for investigating secondary metabolite biosynthesis in Streptomycetes and other filamentous bacteria.
The platform offers potential for significant workload and cost reduction in microbial strain and process development.