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Updated: May 25, 2026

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Published on: April 24, 2018
Aquatic ecotoxicity assessment of a new natural formicide
Renan C Testolin1, Vinícius Tischer, Andre O S Lima
1UNIVALI-Universidade do Vale do Itajaí, Laboratório de Remediação Ambiental, Rua Uruguai, 458, Itajaí, SC 88302-202, Brazil.
Background, Aim And Scope:
Agrochemicals could reach aquatic ecosystems and damage ecosystem functionality. Natural formicide could be an alternative to use in comparison with the more toxic formicides available on the market. Thus, the objective of this study was to assess the ecotoxicity of the new natural formicide Macex® with a battery of classical aquatic ecotoxicity tests.
Material And Methods:
Bacteria (Aliivibrio fischeri), algae (Pseudokirchneriella subcapitata), hydra (Hydra attenuata), daphnids (Daphnia magna), and fish (Danio rerio) tests were performed in accordance with international standardized methodologies.
Results:
In the range of formicide concentrations tested (0.03 to 2.0 g L(-1)) EC(50) values varied from 0.49 to >2.0 g L(-1), with P. subcapitata being the most sensitive species and H. attenuata and D. rerio the most tolerant species to this product in aqueous solutions.
Conclusions:
This new formicide preparation can be classed as a product of low toxicity compared to the aquatic ecotoxicity of the most common commercialized formicides.
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