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[Intraocular penetration of topical norfloxacin in humans. Preliminary study]
A Bron1, M Pechinot, L Chirpaz
1Service d'Ophtalmologie de Dijon, Hôpital Général.
Abstract:
The aim of this bicentric study was to assess the intraocular penetration of topical Norfloxacin. Herein, are only reported the results from one center. Topically applied Norfloxacin 0.3% was given to patients who underwent cataract surgery. The first group received 5 drops between 7.55 A.M. to 8 A.M. and after each hour until cataract surgery. The second one received one drop each quarter from 7 A.M. until the surgery. Aqueous and sera samples were dosed using HPLC. In the first group, the aqueous average was 0.066 mg/l and 0.112 mg/l in the second group. Using a T test and Mann Whitney test, we could not find a significant difference between the two groups.
Insights
This study evaluated topical Norfloxacin penetration in cataract surgery patients. Both dosing regimens showed similar aqueous humor levels, indicating no significant difference in intraocular penetration.
Area of Science:
- Ophthalmology
- Pharmacology
- Drug Delivery
Background:
- Cataract surgery requires effective infection prevention.
- Topical antibiotics are commonly used to prevent postoperative endophthalmitis.
- Understanding intraocular penetration of antibiotics like Norfloxacin is crucial.
Purpose of the Study:
- To assess the intraocular penetration of topical Norfloxacin 0.3% in patients undergoing cataract surgery.
- To compare the efficacy of two different dosing regimens for topical Norfloxacin.
- To determine aqueous humor concentrations of Norfloxacin.
Main Methods:
- A bicentric study was conducted, with results from one center reported.
- Patients received topical Norfloxacin 0.3% via two different dosing schedules before cataract surgery.
- Aqueous humor and serum samples were collected and analyzed using High-Performance Liquid Chromatography (HPLC).
Main Results:
- The average aqueous humor concentration of Norfloxacin was 0.066 mg/l in the first group (frequent drops) and 0.112 mg/l in the second group (less frequent, timed drops).
- Statistical analysis using T test and Mann Whitney test did not reveal a significant difference between the two groups' aqueous humor concentrations.
- Sera samples were also analyzed, though specific results were not detailed in the provided abstract.
Conclusions:
- Both tested dosing regimens of topical Norfloxacin 0.3% achieve measurable concentrations in the aqueous humor.
- There was no statistically significant difference in intraocular penetration between the two dosing strategies.
- Further research may be needed to optimize Norfloxacin dosing for cataract surgery prophylaxis.