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Updated: May 25, 2026

08:57
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
A C-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity--implication for a simple colorimetric
Shizuka Nakayama1, Kevin Roelofs, Vincent T Lee
1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Molecular Biosystems
|January 19, 2012
Abstract:
Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.

