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pJIE137 carrying blaCTX-M-62 is closely related to p271A carrying blaNDM-1

Sally R Partridge1, Ian T Paulsen, Jonathan R Iredell

  • 1Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia. sally.partridge@swahs.health.nsw.gov.au

Antimicrobial Agents and Chemotherapy
|January 19, 2012
PubMed

Insights

Sequencing revealed pJIE137 shares a backbone with p271A, differing in a key regulon region and transposition unit locations. This study details plasmid evolution and antibiotic resistance gene carriage.

Area of Science:

  • Molecular Biology
  • Genomics
  • Plasmid Biology

Background:

  • IncN plasmids are important mobile genetic elements carrying antibiotic resistance genes.
  • Plasmid evolution involves recombination and transposition events that can alter gene content and function.
  • Understanding plasmid diversity is crucial for tracking antimicrobial resistance.

Purpose of the Study:

  • To characterize the complete genome of the pJIE137 plasmid.
  • To compare pJIE137 with related plasmids, such as p271A, to understand their evolutionary relationship.
  • To identify genetic elements responsible for antimicrobial resistance within these plasmids.

Main Methods:

  • Complete plasmid sequencing using next-generation sequencing technologies.
  • Bioinformatic analysis for sequence comparison, gene identification, and structural analysis.
  • Comparative genomics to identify shared and unique regions between plasmids.

Main Results:

  • pJIE137 shares a backbone with p271A, exhibiting ~70% nucleotide identity to IncN plasmids and encoding a novel RepA protein.
  • A region homologous to the IncN CUP regulon is present in pJIE137 but largely absent in p271A, suggesting recombination.
  • Distinct insertion sites for a class 1 In/Tn and ISEcp1-bla(CTX-M-62) transposition unit in pJIE137, and a bla(NDM-1) carrying transposon in p271A were identified.

Conclusions:

  • The study elucidates the genomic structure and evolutionary relationship between pJIE137 and p271A.
  • Recombination events appear to have shaped the IncN CUP regulon region in pJIE137.
  • Differential insertion of transposition units highlights the dynamic nature of plasmid backbones in carrying antibiotic resistance genes.

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