Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytochemistry of esterases.

L Kass

    CRC Critical Reviews in Clinical Laboratory Sciences
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Esterases, enzymes that break ester bonds, are detected in blood cells using synthetic substrates. Fluoride-inhibited nonspecific esterase indicates monocytic origin, while specific esterase marks granulocytic cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The UV filter benzophenone 3, alters early follicular assembly in rat whole ovary cultures.

    Toxicology letters·2019
    Same author

    Effect of magnesium supplementation on blood pressure: a meta-analysis.

    European journal of clinical nutrition·2012
    Same author

    Sex-dependent components of the analgesia produced by athletic competition.

    The journal of pain·2003
    Same author

    Collagen remodelling in the guinea-pig uterine cervix at term is associated with a decrease in progesterone receptor expression.

    Molecular human reproduction·2003
    Same author

    A new stain for identification of avian leukocytes.

    Biotechnic & histochemistry : official publication of the Biological Stain Commission·2002
    Same author

    Relaxin has a minor role in rat mammary gland growth and differentiation during pregnancy.

    Endocrine·2002

    Area of Science:

    • Biochemistry
    • Hematology
    • Enzymology

    Background:

    • Esterases are enzymes catalyzing the hydrolysis of ester bonds.
    • Hematopoietic cells contain various esterase activities.
    • Synthetic substrates are crucial for demonstrating esterase activity in these cells.

    Purpose of the Study:

    • To differentiate hematopoietic cell lineages using esterase activity.
    • To identify specific and nonspecific esterase markers for cell identification.
    • To explore the diagnostic potential of esterase patterns in hematologic disorders.

    Main Methods:

    • Demonstration of esterase activity in hematopoietic cells using alpha naphthyl acetate and alpha naphthyl butyrate for nonspecific esterase.
    • Detection of specific esterase activity using naphthol ASD-chloroacetate.

    Related Experiment Videos

  • Analysis of fluoride inhibition patterns for cell origin determination.
  • Electrophoretic techniques for isoenzymatic analysis.

    • Main Results:

    • Nonspecific esterase activity, inhibited by fluoride, is characteristic of monocytic and reticulum cells.
    • Specific esterase activity, a lysosomal enzyme, serves as a marker for granulocytic cells.
    • Distinct esterase activity patterns are observed in marrow cells from patients with hematologic disorders.

    Conclusions:

    • Specific and nonspecific esterase activities are valuable tools for identifying hematopoietic cell lineages.
    • Esterase enzyme analysis aids in the diagnosis and classification of hematologic malignancies.
    • Isoenzymatic analysis offers further insights into esterase function and cellular origin.