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Updated: May 25, 2026
Multi-scale Analysis of Bacterial Growth Under Stress Treatments
Published on: November 28, 2019
Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in
Maria Grazia Bonomo1, Maria Anna Sico, Simona Grieco
1Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy. mariaanna.sico@unibas.it
Abstract:
Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions.
