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Perturbative forward solver software for small localized fluorophores in tissue.

F Martelli¹, S Del Bianco, P Di Ninni

¹Dipartimento di Fisica e Astronomia dell’Universit`a degli Studi di Firenze,Via G. Sansone 1, 50019 Sesto Fiorentino, Firenze, Italy. fabrizio.martelli@unifi.it

Biomedical Optics Express

|January 19, 2012

Summary

This study introduces novel software for simulating fluorescent biological tissues using the Born approximation. The tool models photon migration and calculates optical properties like reflectance and transmittance.

Keywords:

(170.3660) Light propagation in tissues (170.3880) Medical and biological imaging (170.5280) Photon migration (170.6280) Spectroscopy, fluorescence and luminescence

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Area of Science:

Biomedical Optics
Computational Biology
Photonic Imaging

Background:

Accurate simulation of fluorescence in biological tissues is crucial for diagnostic imaging.
Existing models often struggle with localized fluorophores and complex photon migration.
The Born approximation offers a computationally tractable approach for scattering media.

Purpose of the Study:

To develop and present a forward solver software for simulating fluorescence in biological tissues.
To model photon migration from source to fluorophore and from fluorophore to detector.
To provide calculable optical properties like reflectance, transmittance, and fluence rate.

Main Methods:

Utilized the Born approximation for simulating localized fluorophores in non-fluorescent tissue.
Employed a mathematical model for photon migration in both time and continuous wave (CW) domains.
Developed FORTRAN subroutines for calculating fluorescent signals in infinite and slab geometries.

Main Results:

The proposed software enables simulation of fluorescence emission and photon transport.
Green's functions are used for geometries where analytical solutions are available.
Calculations of reflectance, transmittance, and fluence rate are supported.

Conclusions:

The developed software provides a valuable tool for researchers studying fluorescence in biological tissues.
The FORTRAN subroutines offer practical implementation for specific geometric configurations.
This work advances the simulation capabilities for optical diagnostics and imaging in biology.