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Updated: May 25, 2026
Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Analysis of integrated optofluidic lab-on-a-chip fluorescence biosensor based on transmittance of light through a
Narayan Krishnaswamy1, T Srinivas, G Mohan Rao
1Department of Instrumentation and Applied Physics, Institute of Science, Bangalore 560012, India. narayank@airtelmail.in
Abstract:
Sensitivity analysis is an important aspect to be looked into while designing lab-on-a-chip systems. In this paper we will be showing with appropriate design that the best sensitivity of the fluorescence biosensor is achieved for an optimal width of fluidic gap, corresponding to a particular mode spot size. We will be also showing that the sensitivity of the biosensor is affected by efficiency of light coupling, which is influenced by changes in the width of fluidic gap, refractive index of the fluid and higher order modes.
