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Updated: May 25, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon
Shih-Chung Wei1, Tsung-Liang Chuang, Hui-Hsin Lu
1Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.
Abstract:
Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (~100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400~500 nm spots by using two-photon excitation fluorescence microscopy.
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