Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
08:19

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Published on: June 22, 2020

Autonomic nervous system driven cardiomyocytes in vitro.

Akimasa Takeuchi1, Masahide Mori, Kana Kitagawa

  • 1Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8563, Japan. takeuchi@bmpe.k.u-tokyo.ac.jp

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|January 19, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AtQQS, an Arabidopsis-specific orphan protein, exhibits broad-spectrum antimicrobial activity through membrane disruption.

Biochemical and biophysical research communications·2026
Same author

Therapeutic drug monitoring-guided linezolid salvage therapy for Lacticaseibacillus paracasei infective endocarditis: A case report.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy·2026
Same author

Impact of preexisting interstitial lung disease on pneumonitis and survival during first-line immune checkpoint inhibitor-based therapy in non-small cell lung cancer.

European journal of cancer (Oxford, England : 1990)·2026
Same author

High-definition transcranial random noise stimulation enhances fluid intelligence with increasing cortical excitability.

Journal of neural engineering·2026
Same author

Multi-Omics Integration Reveals Incomplete Reactivation of Developmental Cell-Cycle Programs in Adult Human Infarcted Hearts.

Chemical biology & drug design·2026
Same author

After-effects of parieto-occipital gamma transcranial alternating current stimulation on behavioral performance and neural activity in visuo-spatial attention task.

PeerJ·2026

This study demonstrates that electrical stimulation of rat superior cervical ganglia (SCG) neurons can modulate the beat rate of co-cultured ventricular myocytes (VMs). Stimulation parameters like pulse frequency and number significantly impact cardiomyocyte activity.

Area of Science:

  • Neuroscience
  • Cardiology
  • Bioengineering

Background:

  • Sympathetic nervous system regulation of cardiac function is crucial.
  • Understanding neuron-myocyte communication is vital for cardiac research.
  • Microelectrode-array (MEA) platforms enable precise electrophysiological studies.

Purpose of the Study:

  • To investigate the functional integration of superior cervical ganglia (SCG) neurons with ventricular myocytes (VMs) using a microfluidic MEA system.
  • To determine the effect of electrical stimulation of SCG neurons on VM beating rate.

Main Methods:

  • Co-culture of rat SCG neurons and VMs in a photolithographically fabricated microfluidic chamber on an MEA.
  • Electrical stimulation of SCG neurons using microelectrodes.

More Related Videos

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

Related Experiment Videos

Last Updated: May 25, 2026

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
08:19

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Published on: June 22, 2020

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

  • Recording of evoked responses in VMs.
  • Statistical analysis using two-way ANOVA.

    • Main Results:

    • Successful co-culture and functional connection between SCG neurons and VMs via microconduits.
    • Evoked electrical responses observed in VMs upon SCG neuron stimulation.
    • VM beat rate was significantly modulated by the frequency and number of electrical stimulation pulses applied to SCG neurons.

    Conclusions:

    • The study establishes a novel platform for studying neuron-cardiomyocyte interactions.
    • Electrical stimulation parameters of sympathetic neurons can precisely control cardiomyocyte beating rate.
    • This model holds potential for drug screening and understanding cardiac autonomic regulation.