Green microfluidics made of corn proteins.

Area of Science:

• Materials Science
• Biotechnology
• Microfluidics

Background:

• Petroleum-based polymers like Poly(dimethylsiloxane) are common in microfluidic devices but pose environmental risks due to non-biodegradability.
• Disposable microfluidic applications require sustainable and eco-friendly materials to mitigate pollution.

Purpose of the Study:

• To fabricate biodegradable microfluidic devices using zein, a biodegradable protein from corn.
• To evaluate the performance and bonding strength of zein-based microfluidic devices for disposable applications.

Main Methods:

• Zein films were fabricated and micro-features (chambers, channels) were replicated using stereolithography and soft lithography.
• Enclosed zein microfluidic devices were created by vapor deposition bonding to glass substrates.
• Device performance was assessed through fluid flow tests, leakage/distortion analysis, and optical clarity/fluorescent imaging.

Main Results:

• Zein microfluidic devices exhibited bonding strength exceeding zein film tensile strength and hydraulic pressure limits.
• Fluid flow in complex, large-area designs showed no leakage or distortion.
• High optical clarity and successful fluorescent imaging of micro-particles and Rhodamine B were demonstrated.

Conclusions:

• Zein microfluidic devices offer a viable, biodegradable, and biocompatible alternative for disposable microfluidics.
• Fabrication utilizes existing techniques, enabling scalable and environmentally friendly micro-chip production.
• These devices support health and environmental applications requiring truly disposable microfluidic systems.