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Related Experiment Video

Updated: May 25, 2026

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

Towards a cellular multi-parameter analysis platform: fluorescence in situ hybridization (FISH) on microhole-array

Christian M Kurz¹, Stefan V D Moosdijk, Hagen Thielecke

¹Department Biomedical Microsystems, Fraunhofer Insitute for Biomedical Engineering.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|January 19, 2012

Summary

We enhanced a chip platform for fluorescence in situ hybridization (FISH) diagnostics, reducing assay costs by 5x. This innovation streamlines DNA sequence analysis for improved diagnostic efficiency.

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Area of Science:

Biotechnology
Medical Diagnostics
Cell Biology

Background:

Highly-sensitive cellular multi-parameter analysis systems are crucial for diagnostics.
Existing methods like fluorescence in situ hybridization (FISH) are labor- and cost-intensive.

Purpose of the Study:

To improve a previously developed chip platform by integrating fluorescence in situ hybridization (FISH).
To reduce assay consumption and labor time for FISH analysis.
To enable simultaneous application of non-invasive spectroscopic methods.

Main Methods:

Fabrication of microhole chips using semiconductor technology on a silicon wafer with a silicon nitride layer.
Arraying human retina pigment epithelia (ARPE-19) cells on 5-μm holes using negative differential pressure.
Application of the FISH protocol to positioned cells.
Development of LabView software for automated analysis of FISH results.

Main Results:

Reduced FISH assay consumption by a factor of 5 compared to standard protocols.
Successfully arrayed and analyzed cells on the microhole chip platform.
Developed automated software for counting FISH dots and measuring distances.
Demonstrated the potential for integrating Raman and impedance spectroscopy during cell incubation.

Conclusions:

The improved chip platform significantly reduces assay consumption and labor time for FISH analysis.
The platform offers a streamlined and potentially more cost-effective approach to genetic diagnostics.
The system allows for simultaneous application of other analytical techniques, enhancing its utility.