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Related Experiment Video

Updated: May 25, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

A robust microfluidic in vitro cell perifusion system.

Christina Morris1, Dylan J Banks, Lukasz Gaweda

  • 1The Centre for Biologically-Inspired Technology, Bessemer Building, Level 4, Imperial College London, South Kensington Campus, London SW7 2AZ. christina.morris04@imperial.ac.uk

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|January 19, 2012
PubMed
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A new microfluidic perifusion device enhances cell secretion studies by concentrating samples. This innovation enables direct mass spectrometry measurement of islet glucagon from pancreatic tissue.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • In vitro cell secretion studies are crucial for understanding tissue function.
  • Standard assays often lack the sensitivity for detecting low-abundance secreted molecules.
  • Improving sample concentration and physiological relevance in perifusion systems is needed.

Purpose of the Study:

  • To develop and validate a robust microfluidic cell perifusion device for enhanced in vitro primary tissue cell secretion analysis.
  • To increase the sample concentration to perifusion volume ratio compared to traditional methods.
  • To enable the direct measurement of specific secreted biomolecules using mass spectrometry.

Main Methods:

  • Fabrication of a microfluidic device using standard machining techniques (drilling, milling).

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Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
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  • Implementation of perifusion with physiologically relevant flow rates, pressures, and temperature.
  • Application of the device for hormone perifusion experiments on primary mouse pancreatic tissue.

    • Main Results:

    • The microfluidic device achieved an order of magnitude increase in sample concentration compared to multi-well plates.
    • Cell secretion concentrations were increased by up to a factor of 20.
    • Enabled the first direct measurement of islet glucagon using mass spectrometry.

    Conclusions:

    • The developed microfluidic perifusion device is robust, cost-effective, and easily replicable.
    • This system significantly enhances the sensitivity of in vitro cell secretion studies.
    • The device facilitates novel direct measurements of key biomarkers like islet glucagon.