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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

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Published on: August 16, 2012

Optofluidic on-chip tomography.

Serhan O Isikman1, Waheb Bishara, Hongying Zhu

  • 1Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA. sisikman@ucla.edu

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|January 19, 2012
PubMed
Summary
This summary is machine-generated.

Optofluidic tomography was demonstrated for the first time, enabling 3D imaging of micro-objects. This technique uses lensfree holograms recorded from flowing micro-objects for on-chip imaging applications.

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Area of Science:

  • Optics
  • Microfluidics
  • Imaging Science

Background:

  • Microfluidic devices enable precise control and manipulation of small fluid volumes.
  • Tomography is a technique used for reconstructing 3D images from a series of 2D projections.
  • Lensfree imaging offers a simplified optical setup by eliminating the need for objective lenses.

Purpose of the Study:

  • To present the first demonstration of optofluidic tomography.
  • To develop a method for 3D imaging of micro-objects in flow.
  • To achieve on-chip sectional imaging using microfluidic and holographic principles.

Main Methods:

  • Partially coherent illumination was used to record holograms of micro-objects.
  • Holograms were captured at multiple viewing angles as objects flowed through a microfluidic channel.
  • A microfluidic chip was integrated with an opto-electronic sensor array for direct hologram recording.
  • Digital processing of lensfree holograms was performed to compute tomograms.
  • Pixel super-resolution techniques were applied for enhanced tomogram reconstruction.

Main Results:

  • Successful demonstration of optofluidic tomography for micro-objects.
  • Acquisition of multi-angle holograms of flowing micro-objects.
  • Generation of pixel super-resolved tomograms from lensfree holograms.
  • Achieved sectional optofluidic imaging directly on a chip.

Conclusions:

  • Optofluidic tomography is a viable technique for 3D imaging of micro-objects in microfluidic systems.
  • The developed method allows for non-invasive, on-chip tomographic imaging.
  • This technology has potential applications in microfluidic analysis and diagnostics.