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[Analysis of the changes of amide A in rabbit heart after death by FTIR spectroscopy]
Hai-Dong Zhang1, Tian-Tong Yang, Na Zheng
1Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing 100040, China. zhd_zzw@126.com
Objective:
To explore the relationship of changes of amide A in rabbit heart and the postmortem interval (PMI) by FTIR spectroscopy technique.
Methods:
Thirty-two rabbits were sacrificed and the hearts were sampled at 20 degrees C within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system.
Results:
The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios (y) of amide A (positive and negative area) and PMI(x). The regression equation was y = 0.001x2-0.038x + 0.747(R2 = 0.940).
Conclusion:
The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.
