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Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Potentiometry: Overview01:06

Potentiometry: Overview

Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as the...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...

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Related Experiment Video

Updated: May 25, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Label-free and substrate-free potentiometric aptasensing using polycation-sensitive membrane electrodes.

Jiawang Ding1, Yan Chen, Xuewei Wang

  • 1Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, P. R. China.

Analytical Chemistry
|January 24, 2012
PubMed
Summary

This study introduces a novel potentiometric label-free and substrate-free aptasensing strategy. It enables sensitive detection of molecules like adenosine triphosphate (ATP) in solutions and cells without complex steps.

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Last Updated: May 25, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

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Published on: March 21, 2018

Introduction to Solid Supported Membrane Based Electrophysiology
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Published on: May 11, 2013

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
08:03

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Area of Science:

  • Electrochemistry
  • Biosensing
  • Molecular Biology

Background:

  • Existing aptasensors often require labeling, separation, or immobilization steps, adding complexity.
  • Label-free and substrate-free (LFSF) aptasensing aims to simplify detection methods.
  • Electrochemical transduction offers a sensitive and potentially cost-effective alternative to optical detection.

Purpose of the Study:

  • To develop a novel potentiometric label-free and substrate-free (LFSF) aptasensing strategy.
  • To demonstrate the detection of target molecules based on aptamer conformational changes.
  • To apply the strategy for determining adenosine triphosphate (ATP) in biological samples.

Main Methods:

  • Utilized aptamer-target binding to induce a conformational change in the aptamer.
  • Employed a potentiometric method using a polycation-sensitive membrane electrode to monitor changes.
  • Detected adenosine triphosphate (ATP) as a model analyte in homogeneous solutions and HeLa cells.

Main Results:

  • Achieved label-free and substrate-free (LFSF) potentiometric detection of ATP down to the submicromolar range.
  • Demonstrated successful application in determining ATP concentrations within HeLa cells.
  • Showcased electrochemical transduction of aptamer-target binding events in a homogeneous solution.

Conclusions:

  • The proposed potentiometric LFSF aptasensing strategy offers a simplified and sensitive approach for biosensing.
  • This method effectively utilizes target-induced aptamer conformational changes for signal generation.
  • The strategy provides a foundation for developing new electrochemical sensors for various analytes involving conformational changes.