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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...

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PET imaging for gene & cell therapy.

Sara A Collins1, Kei Hiraoka, Akihito Inagaki

  • 1UCLA School of Medicine, Los Angeles, USA.

Current Gene Therapy
|January 24, 2012
PubMed
Summary
This summary is machine-generated.

Positron Emission Tomography (PET) offers a sensitive, non-invasive method for monitoring gene therapy delivery and expression. This technique enables repeated in vivo imaging, crucial for improving clinical protocols and validating treatment efficacy.

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Area of Science:

  • Molecular Imaging
  • Gene Therapy
  • Biomedical Engineering

Background:

  • Growing interest in gene therapy necessitates reliable methods for monitoring treatment in patients.
  • Non-invasive imaging is crucial for repeated validation of gene delivery and expression post-vector administration.
  • Positron Emission Tomography (PET) is a sensitive imaging modality adapted for both pre-clinical and clinical gene therapy studies.

Purpose of the Study:

  • To review the principles and applications of PET imaging for monitoring gene and cell therapy.
  • To compare PET with other imaging modalities for gene therapy assessment.
  • To highlight the clinical applicability of PET in analyzing gene therapy.

Main Methods:

  • Utilizes positron-emitting molecular probes for in vivo detection of transgene activity.
  • Employs radiolabeled substrates interacting with specific transgene proteins.
  • Reviews existing literature on PET imaging in gene and cell therapy.

Main Results:

  • Reporter genes suitable for imaging vector-mediated gene delivery and expression have been identified.
  • PET enables non-invasive analysis of transgene location, level, and kinetics.
  • PET demonstrates optimal sensitivity and quantitation for in vivo gene expression measurements.

Conclusions:

  • PET imaging is the most clinically applicable modality for analyzing gene therapy due to its sensitivity, quantitation, and widespread availability.
  • PET facilitates non-invasive, repeated monitoring essential for refining gene therapy protocols.
  • The review underscores PET's significant role in advancing both pre-clinical and clinical gene therapy research.