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08:09
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Enterovirus 71 viral capsid protein linear epitopes: identification and characterization
Fan Gao1, Yi-Ping Wang, Qun-Ying Mao
1National Institutes for Food and Drug Control, Beijing, China.
Virology Journal
|January 24, 2012
Summary
This study identified human immune responses to enterovirus 71 (EV71) capsid proteins. IgM epitopes were found on VP2 and VP3 during acute infection, while a single IgG epitope on VP1 appeared in recovery.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Enterovirus 71 (EV71) causes significant public health concern.
- Understanding the human humoral immune response to EV71 is crucial for vaccine development and diagnostics.
Purpose of the Study:
- To characterize the human humoral immune response against EV71 infection.
- To map human epitopes on the viral capsid proteins (VP1, VP2, VP3).
Main Methods:
- Synthesized 256 overlapping peptides spanning EV71 capsid proteins.
- Used indirect ELISA to detect anti-EV71 IgM and IgG in sera from infected children.
- Screened peptides using rabbit anti-EV71 IgG.
Main Results:
- Identified 10 human IgM epitopes in acute phase sera, primarily on VP2 and VP3.
- Detected one human IgG epitope on VP1 in recovery phase sera.
- Mapped four rabbit IgG epitopes to VP1.
Conclusions:
- Human IgM responses target VP2 and VP3 during acute EV71 infection.
- A distinct IgG epitope on VP1 emerges during the recovery phase.
- Dynamic humoral immune responses have implications for EV71 vaccine immunogenicity and diagnostic reagent development.
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