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Updated: May 25, 2026
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
Published on: June 18, 2015
Microbial transformation of deoxyandrographolide and their inhibitory activity on LPS-induced NO production in RAW
Sa Deng1, Bao Jing Zhang, Chang Yuan Wang
1School of Pharmaceutical Sciences, Dalian Medical University, Dalian 116044, PR China.
Abstract:
A series of analogues of deoxyandrographolide (1) transformed by Cunninghamella blakesleana AS 3.2004 were isolated and identified by spectral methods including 2D NMR. Among them, 3-oxo-17,19-dihydroxy-7,13-ent-labdadien-15,16-olide (9), 3-oxo-19-hydroxy-1,13-ent-labdadien-15,16-olide (16), 3-oxo-1β-hydroxy-14-deoxy-andrographolide (17) and 3-oxo-2β-hydroxy-14-deoxyandrographolide (18) are new compounds. And their structure-activity relationships (SAR) of inhibitory activity on LPS-induced NO production in RAW 264.7 macrophage cells were also discussed.
