Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prognostic, diagnostic and therapeutic value of the cancer tubulin code.

Cancer metastasis reviews·2026

Same author

Condensin I but not Condensin II is crucial for mitotic chromosome mechanics.

Nature communications·2026

Same author

Parthenolide disrupts mitosis by inhibiting ZNF207/BUGZ-promoted kinetochore-microtubule attachment.

The EMBO journal·2025

Same author

Mechanistic insight into anaphase bridge signaling to the abscission checkpoint.

The EMBO journal·2025

Same author

Proximity-based activation of AURORA A by MPS1 potentiates error correction.

Current biology : CB·2025

Same author

How does the tubulin code facilitate directed cell migration?

Biochemical Society transactions·2025

Search research articles

Related Experiment Video

Updated: May 25, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Fluorescent speckle microscopy in cultured cells.

Marin Barisic¹, António J Pereira, Helder Maiato

¹Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.

Methods in Enzymology

|January 24, 2012

Summary

Fluorescent speckle microscopy (FSM) visualizes macromolecular dynamics by observing fluorescently labeled molecules within cells. This technique has advanced significantly, offering improved methods for studying cellular processes.

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Related Experiment Videos

Last Updated: May 25, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Area of Science:

Cellular dynamics and molecular biology
Advanced microscopy techniques

Background:

Fluorescent speckle microscopy (FSM) has been a key technique for over a decade.
It investigates macromolecular dynamics like microtubule and actin filament assembly.

Purpose of the Study:

To describe the current fluorescent speckle microscopy (FSM) setup.
To detail experimental approaches for using FSM in cultured cells.
To discuss the challenges and future of FSM.

Main Methods:

FSM relies on a low ratio of fluorescently labeled to endogenous molecules for contrast.
Techniques include microinjection or expressing limited amounts of labeled subunits.
Improvements involve sensitive cameras, spinning-disk confocal units, and specialized image analysis algorithms.

Main Results:

FSM enables visualization of dynamic processes like microtubule flux and protein assembly.
The technique's contrast relies on the non-uniform distribution of labeled subunits.
Advancements have enhanced image quality and analysis capabilities.

Conclusions:

FSM is a powerful technique for studying cellular dynamics.
Ongoing improvements in hardware and software continue to enhance its utility.
Future challenges involve further refining the technique for even greater insights.