Related Experiment Video
Updated: May 25, 2026

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
Green-to-red photoconvertible mEosFP-aided live imaging in plants
Jaideep Mathur1, Sarah Griffiths, Kiah Barton
1Department of Molecular and Cellular Biology, Laboratory of Plant Development and Interactions, University of Guelph, Guelph, Canada.
Abstract:
Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging.
