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Updated: May 25, 2026

Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

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Corticosteroids and local anesthetics decrease positive effects of platelet-rich plasma: an in vitro study on human

Bradley Carofino¹, David M Chowaniec, Mary Beth McCarthy

¹Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.

Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association

|January 24, 2012

Summary

Mixing anesthetics or corticosteroids with platelet-rich plasma (PRP) significantly reduces tenocyte proliferation and viability in vitro. Careful use is advised to preserve PRP

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Last Updated: May 25, 2026

Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

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Published on: September 29, 2018

Area of Science:

Orthopedics
Cell Biology
Biomaterials Science

Background:

Platelet-rich plasma (PRP) is utilized for tendon healing.
The impact of combining PRP with anesthetics or corticosteroids on tenocytes is not fully understood.

Purpose of the Study:

To investigate the in vitro effects of anesthetics and corticosteroids mixed with PRP on human tenocytes.
To assess the impact on tenocyte proliferation and viability.

Main Methods:

Human tenocytes were cultured with PRP obtained via double and single spin protocols.
Cells were exposed to PRP alone, or in combination with lidocaine, bupivacaine, or methylprednisolone.
Cell proliferation and viability were measured using radioactive thymidine and luminescence assays.

Main Results:

Anesthetics (lidocaine, bupivacaine) and methylprednisolone significantly reduced tenocyte proliferation compared to controls.
Lidocaine and bupivacaine showed greater inhibition of proliferation than methylprednisolone.
Cell viability was significantly decreased in the presence of anesthetics or methylprednisolone.

Conclusions:

Combining anesthetics or corticosteroids with PRP significantly impairs tenocyte proliferation and viability in vitro.
This suggests that co-administration may compromise the therapeutic benefits of PRP for tendon injuries.
Judicious use of these agents with PRP is recommended to maintain efficacy in tendon repair.