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Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

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Combining microdilution with MicroResp™: microbial substrate utilization, antimicrobial susceptibility and

Sigrid Drage¹, Doris Engelmeier, Gert Bachmann

¹Department of Chemical Ecology and Ecosystem Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.

Journal of Microbiological Methods

|January 24, 2012

Summary

This study modified the MicroResp™ system to assess microbial growth efficiency and antimicrobial susceptibility. The enhanced system reveals dose-response effects and hormesis in microbial communities, aiding ecological and pharmacological research.

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Area of Science:

Microbiology
Ecology
Pharmacology

Background:

Traditional studies assess microbial susceptibility to drugs or substrate utilization by communities.
The MicroResp™ system is a tool for soil microbial community analysis.
Understanding microbial growth efficiency and responses to various compounds is crucial.

Purpose of the Study:

To modify the MicroResp™ system for exploring microbial growth efficiency in a concentration-dependent manner.
To investigate dose-response effects, including hormesis, in microbial communities.
To analyze microbial growth, respiration, and efficiency using multivariate methods.

Main Methods:

Modification of the MicroResp™ system for microdilution assays.
Turbidimetric growth assessment and colorimetric indicator plates (cresol red agar).
Curve fitting using nonlinear models to detect hormetic effects (U-shaped and inverse U-shaped).
Multivariate analyses of growth, respiration, and growth efficiency.

Main Results:

The modified MicroResp™ system effectively explores microbial growth efficiency against varying concentrations of substrates and toxic chemicals.
Dose-response curves revealed hormetic effects, indicating stimulation at low doses and inhibition at high doses.
Multivariate analysis provided insights into the relationships between microbial growth, respiration, and efficiency.

Conclusions:

The modified MicroResp™ system is a valuable tool for studying microbial ecophysiology and antimicrobial susceptibility.
The detection of hormesis highlights complex microbial responses to environmental factors and chemicals.
This approach enhances our understanding of microbial community dynamics and interactions.