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Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

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Published on: December 13, 2016

Anion deposition into ferritin.

Robert J Hilton¹, Bo Zhang, L Naomi Martineau

¹Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, United States.

Journal of Inorganic Biochemistry

|January 24, 2012

Summary

This summary is machine-generated.

Ferritin sequesters anions during iron core reduction to balance charge, expelling them upon iron re-oxidation. This anion transport mechanism is crucial for maintaining ferritin

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Area of Science:

Biochemistry
Structural Biology
Biomineralization

Background:

Ferritin is a protein shell that stores iron.
Iron storage involves redox reactions within the ferritin core.
Charge balance is critical during iron core reduction.

Purpose of the Study:

To investigate anion translocation across the ferritin shell during iron core reduction.
To understand the mechanism of charge balancing within ferritin.
To determine the selectivity of ferritin for different anions.

Main Methods:

Monitoring anion sequestration using ion-selective electrodes.
Quantifying anion and iron concentrations via inductively coupled plasma emission.
Analyzing elemental composition with energy-dispersive X-ray spectroscopy.
Visualizing iron sequestration using electron microscopy and column chromatography.

Main Results:

Anions translocate into ferritin during iron core reduction to neutralize released hydroxyl ions.
Smaller halides show greater accumulation than larger halides due to steric hindrance.
Oxoanion accumulation is not size or charge selective, with vanadate and molybdate accumulating most.
Reduced iron (Fe(II)) remains stable within ferritin.
Anions are expelled upon iron core re-oxidation.

Conclusions:

Anion transport across the ferritin shell is a key mechanism for maintaining charge balance.
Ferritin exhibits size-dependent selectivity for halide anions.
The process is reversible, with anions released upon iron re-oxidation.