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Related Experiment Video

Updated: May 25, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

A novel method for detecting antigen-specific human regulatory T cells.

Lisa M Ebert1, Sarah E MacRaild, Ian D Davis

  • 1Ludwig Institute for Cancer Research (Melbourne-Austin Branch), Melbourne, Australia.

Journal of Immunological Methods
|January 24, 2012
PubMed
Summary

Researchers developed a new assay to detect rare human regulatory T cells (Treg) specific to antigens. This method uses CD3 down-regulation as a marker for antigen recognition, advancing Treg research.

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Area of Science:

  • Immunology
  • Cellular Biology
  • T Cell Research

Background:

  • Defining antigen specificity for FoxP3(+) regulatory T cells (Treg) is challenging.
  • Existing assays for Treg specificity are limited.
  • Understanding Treg recognition is crucial for immunotherapy.

Purpose of the Study:

  • To develop a novel assay for detecting human Treg specific to peptide antigens.
  • To establish a reliable method for identifying rare antigen-specific Treg.
  • To facilitate the study of Treg responses in various contexts, including cancer.

Main Methods:

  • Developed a new assay utilizing down-regulation of surface CD3 as a readout for antigen recognition.
  • Optimized culture conditions and re-stimulation time for Treg detection.

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Last Updated: May 25, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Generation of Human Chimeric Antigen Receptor Regulatory T Cells
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Generation of Human Chimeric Antigen Receptor Regulatory T Cells

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  • Applied the assay to detect human Treg specific to peptide antigens.

    • Main Results:

    • Successfully developed and optimized a novel assay for detecting human Treg specificity.
    • The assay allows for the detection of rare Treg populations.
    • Demonstrated the ability to identify Treg specific to tumor antigens.

    Conclusions:

    • The novel CD3 down-regulation assay provides a sensitive method for identifying antigen-specific Treg.
    • This assay overcomes limitations of previous methods for determining Treg specificity.
    • The findings have implications for understanding immune responses and developing Treg-based therapies.