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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Published on: October 18, 2013

Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture.

Nadin Rohland1, David Reich

  • 1Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. nrohland@genetics.med.harvard.edu.

Genome Research
|January 24, 2012
PubMed
Summary
This summary is machine-generated.

We developed a cost-effective DNA sequencing library preparation method, reducing costs to $15 per sample and enabling high-throughput processing. This approach accelerates genomic studies, including large-scale cancer research.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • DNA sequencing costs have decreased significantly due to technological advancements.
  • Sample preparation remains a bottleneck in terms of time and cost for many genomic experiments.
  • Efficient and scalable methods are crucial for large-scale genetic studies.

Purpose of the Study:

  • To present a novel, cost-effective, and time-efficient approach for DNA sequencing library preparation.
  • To demonstrate the utility of this method for both low-pass whole-genome sequencing and targeted enrichment.
  • To validate the method's effectiveness on a large cohort of samples from a prostate cancer study.

Main Methods:

  • Developed a protocol to prepare 192 DNA sequencing libraries within one day of technician time.
  • Achieved a cost of approximately $15 per sample for library preparation.
  • Integrated individual barcoding for pooling up to 100 samples per library.
  • Assessed library performance for whole-genome sequencing and targeted enrichment.

Main Results:

  • Successfully prepared 192 sequencing libraries in a single day at a low cost per sample.
  • Demonstrated high efficiency for low-pass whole-genome sequencing.
  • Showcased effective simultaneous targeted enrichment in pooled, barcoded samples.
  • Validated the approach on approximately 2000 samples from a prostate cancer cohort.

Conclusions:

  • The presented method significantly reduces the cost and time associated with DNA sequencing library preparation.
  • This approach enables high-throughput genomic analyses, including pooled targeted enrichment.
  • The method is effective for large-scale studies, such as population-based cancer research.