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Published on: October 2, 2012
Increased furan tolerance in Escherichia coli due to a cryptic ucpA gene
Xuan Wang1, Elliot N Miller, Lorraine P Yomano
1Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA.
Applied and Environmental Microbiology
|January 24, 2012
Abstract:
Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.

