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Development of 15 novel microsatellite markers from cellulose synthase genes in Populus tomentosa (Salicaceae)
Qing-Zhang Du1, De-Qiang Zhang, Bai-Lian Li
1National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, People's Republic of China.
Premise Of The Study:
Microsatellite markers from cellulose synthase genes were developed for the Chinese white poplar, Populus tomentosa, to investigate the genetic diversity of wild germplasm resources and to further identify favorable alleles significantly associated with wood cellulose content.
Methods And Results:
Fifteen microsatellite markers were developed in P. tomentosa by deep sequencing of cellulose synthase genes. Polymorphisms were evaluated in 460 individuals from three climatic regions of P. tomentosa, and all 15 markers revealed polymorphic variation. The number of alleles per locus ranged from two to nine with an average of 4.3; the observed and expected heterozygosity per locus varied from 0.029 to 0.962 and from 0.051 to 0.713, respectively.
Conclusions:
These polymorphic markers will potentially be useful for genetic mapping and in molecular breeding for improvement of wood fiber traits in Populus.
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