Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genomic advances in orphan and underutilized Brassicaceae crops and their wild relatives.

Frontiers in plant science·2026

Same author

Digital Technology Use for Health and eHealth Literacy in a Very Remote Aboriginal Community in the Northern Territory, Australia: A Community-Based Study.

The Australian journal of rural health·2026

Same author

Dormancy regulon reduction was pivotal to the evolution of Mycobacterium tuberculosis.

Nature communications·2026

Same author

Establishing the Standardized EMS Metrics for Survival in Transfusion and Advanced Resuscitation: the SEMSTAR project.

Trauma surgery & acute care open·2026

Same author

Dissection of local haplotype diversity at soybean rust loci reveals resistance-associated and context-dependent variation patterns in diverse germplasm.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same author

Accessing crop genetic diversity via pangenomics.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Search research articles

Related Experiment Video

Updated: May 25, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Next-generation sequencing applications for wheat crop improvement.

Paul J Berkman¹, Kaitao Lai, Michal T Lorenc

¹University of Queensland, School of Agriculture and Food Sciences and Australian Centre for Plant Functional Genomics, Brisbane, QLD 4072, Australia.

American Journal of Botany

|January 24, 2012

Summary

Next-generation sequencing (NGS) advances wheat breeding for improved crop yields. These technologies enable faster genome analysis, identifying genetic variations crucial for enhancing wheat production to meet global food demands.

More Related Videos

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

Related Experiment Videos

Last Updated: May 25, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

Area of Science:

Plant biology
Genomics
Agricultural science

Background:

Bread wheat (Triticum aestivum) is a vital global food source, providing 20% of daily calories.
Wheat yield increases have slowed, necessitating innovation to meet future population demands.
Selective breeding and agronomic practices have historically driven yield improvements.

Purpose of the Study:

To review the application of next-generation sequencing (NGS) in wheat improvement.
To highlight the impact of NGS on understanding the wheat genome.
To discuss challenges and opportunities in using NGS for crop enhancement.

Main Methods:

Overview of wheat genome sequencing and its complexities.
Description of various next-generation sequencing technologies.
Analysis of NGS applications in identifying genetic variation and gene expression.

Main Results:

NGS technologies significantly reduce the cost and time for genome sequencing.
NGS enables detailed assessment of gene structure and expression in wheat.
NGS facilitates the identification of heritable variations linked to agronomic traits.

Conclusions:

NGS is revolutionizing plant biology and offers powerful tools for wheat crop improvement.
Addressing challenges in NGS application is key to unlocking its full potential in wheat breeding.
The integration of NGS is critical for accelerating the development of higher-yielding wheat varieties.