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Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features.

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Updated: May 25, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Targeted sequence capture as a powerful tool for evolutionary analysis.

Corrinne E Grover1, Armel Salmon, Jonathan F Wendel

  • 1Department of Ecology, Evolution, & Organismal Biology, Iowa State University, Ames, Iowa 50011, USA. corrinne@iastate.edu

American Journal of Botany
|January 24, 2012
PubMed
Summary
This summary is machine-generated.

Targeted sequence enrichment combined with next-generation sequencing (NGS) offers ecologists and evolutionary biologists an efficient method to analyze genomic regions. This approach enhances data generation for diverse research questions, especially for species with limited genomic resources.

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Published on: October 18, 2013

Area of Science:

  • Ecology
  • Evolutionary Biology
  • Genomics

Background:

  • Next-generation sequencing (NGS) has transformed biological research, enabling large-scale data generation.
  • While NGS is powerful, whole genome sequencing is often excessive for specific ecological and evolutionary questions.
  • Many taxa lack extensive genomic resources, limiting traditional large-scale genomic studies.

Purpose of the Study:

  • To introduce targeted sequence enrichment as a powerful strategy for ecological and evolutionary research.
  • To highlight the benefits of combining targeted sequence enrichment with NGS for data generation.
  • To demonstrate the applicability of this approach for various research questions across different taxonomic levels.

Main Methods:

  • Utilizing targeted sequence enrichment techniques to isolate specific genes or genomic regions.
  • Applying next-generation sequencing (NGS) to analyze the enriched DNA samples.
  • Analyzing data to address ecological and evolutionary questions at infra- and interspecific levels.

Main Results:

  • Targeted sequence enrichment coupled with NGS provides an efficient and effective method for data generation.
  • This approach allows for the analysis of a few to thousands of genes/genomic regions from numerous samples.
  • The technology is applicable to a wide range of research questions, including parentage, gene flow, divergence, and phylogenetics.

Conclusions:

  • Targeted sequence enrichment significantly enhances the ability of ecologists and evolutionary biologists to address key research questions.
  • This method is particularly valuable for studies involving taxa with limited genomic resources.
  • The combination of targeted enrichment and NGS opens new research avenues in ecology and evolution.