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Related Experiment Video

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Fast and simple LC-MS/MS method for quantifying plasma voriconazole.

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Summary
This summary is machine-generated.

A new LC-MS/MS method enables rapid and simple quantification of voriconazole in plasma. This technique simplifies clinical routine analysis, reducing workload and costs.

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Area of Science:

  • Analytical Chemistry
  • Clinical Chemistry
  • Pharmacokinetics

Background:

  • Growing evidence highlights the necessity of therapeutic drug monitoring for voriconazole.
  • Accurate plasma concentration measurements are crucial for optimizing voriconazole therapy.

Purpose of the Study:

  • To develop a rapid and straightforward liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.
  • To quantify voriconazole concentrations in human plasma samples.

Main Methods:

  • Utilized protein precipitation for sample pretreatment.
  • Employed a 2-minute analysis time on an LC-MS/MS system.
  • Quantification achieved using calibration curves and a predefined factor.

Main Results:

  • The method demonstrated excellent precision (CV 2.8–11.7%) and accuracy (89.0–109.0%).
  • Linearity was established across a wide concentration range (0.06–20.0 mg/L).
  • Analysis time was reduced to 2 minutes per sample.

Conclusions:

  • The developed LC-MS/MS method is suitable for routine clinical analysis of voriconazole.
  • Quantification using a predefined factor streamlines the process, reducing costs and turnaround time.