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Related Experiment Video

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Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Published on: April 5, 2019

Phenotypic analysis of pneumococcal polysaccharide-specific B cells.

Noor Khaskhely¹, Jason Mosakowski, Rebecca S Thompson

¹Department of Medicine, University of Toledo, Toledo, OH 43614, USA.

Journal of Immunology (Baltimore, Md. : 1950)

|January 25, 2012

Summary

This study reveals that IgM memory B cells play a key role in the immune response to pneumococcal polysaccharides. Post-vaccination, these cells, identified by CD27(+)IgM(+), become dominant among PPS-specific B cells.

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Area of Science:

Immunology
Vaccinology

Background:

The precise B cell phenotype responsible for anti-pneumococcal polysaccharide antibody production remains unclear.
Individuals with poor responses to pneumococcal polysaccharide vaccines often lack both IgM memory and switched memory B cells.

Purpose of the Study:

To characterize the phenotype of pneumococcal polysaccharide (PPS)-specific B cells before and after immunization.
To elucidate the role of different B cell subsets in the humoral immune response to PPS.

Main Methods:

Fluorescently labeled PPS14 and PPS23F were used to identify and characterize PPS-specific B cells via flow cytometry.
Phenotypic analysis of B cell populations (naive, IgM memory, switched memory) was performed on samples from young adults pre- and post-Pneumovax immunization.

Main Results:

PPS-specific B cells obtained post-immunization were predominantly IgM memory cells (CD27(+)IgM(+)), significantly higher than in unselected B cells.
Pre-immunization PPS-specific B cells were mainly naive (CD27(-)).
No significant difference in switched memory B cell (CD27(+)IgM(-)) populations was observed between groups.

Conclusions:

IgM memory B cells (CD27(+)IgM(+)) play a dominant role in the immune response to pneumococcal polysaccharides.
The findings clarify the B cell subset involved in effective anti-PPS immunity.