Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

Overview
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Studying Early Life Live-Attenuated influenza virus immune Responses (STELLAR): study protocol for an exploratory observational study of the nasal mucosal and systemic immune response in healthy children given an intranasal live-attenuated influenza vaccine.

BMJ open·2026
Same author

Keeping up with CFTR modulator eligibility.

Paediatric respiratory reviews·2026
Same author

Dimensional Associations Between Conduct Problems and Brain Structure Across 18 International Cohorts in ENIGMA.

Journal of the American Academy of Child and Adolescent Psychiatry·2026
Same author

Hidden harm: Quantifying occupational injury under-reporting in veterinary workplaces through modified capture-recapture analysis.

Veterinary journal (London, England : 1997)·2026
Same author

Surface freshening in the subpolar North Atlantic sustaining the weakened AMOC during the late Younger Dryas.

Science advances·2026
Same author

Operationalizing Decentralized Clinical Trials: Technology Insights from the Trials@Home RADIAL Proof-of-Concept Trial.

Clinical pharmacology and therapeutics·2025
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: May 25, 2026

Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Loop-mediated amplification accelerated by stem primers.

Olga Gandelman1, Rebecca Jackson, Guy Kiddle

  • 1Lumora Ltd., Unit 4, Cambridgeshire Business Park, Bartholomews Walk, Ely, Cambridgeshire CB7 4EA, UK. o.gandelman@lumora.co.uk

International Journal of Molecular Sciences
|January 25, 2012
PubMed
Summary
This summary is machine-generated.

Loop-mediated amplification (LAMP) primer design is challenging. A new STEM-LAMP method using stem primers offers improved speed, sensitivity, and flexibility for molecular diagnostics.

Keywords:
Clostridium difficileHIVListeria monocytogenesin vitro diagnosticsisothermal amplificationloop-mediated amplificationstem primers

More Related Videos

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Related Experiment Videos

Last Updated: May 25, 2026

Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Area of Science:

  • Molecular Biology
  • Biotechnology
  • In Vitro Diagnostics

Background:

  • Isothermal nucleic acid amplifications (iNAATs) are vital alternatives to PCR in molecular diagnostics.
  • Loop-mediated amplification (LAMP) is a popular iNAAT due to its simple hardware requirements and PCR-equivalent performance.
  • LAMP's widespread application is hindered by complex primer design involving six primers and eight specific priming sites.

Purpose of the Study:

  • To address the limitations of traditional LAMP primer design.
  • To introduce and evaluate an alternative approach using stem primers (STEM-LAMP) instead of loop primers.
  • To demonstrate the utility of STEM-LAMP for detecting Clostridium difficile, Listeria monocytogenes, and HIV.

Main Methods:

  • Developed a novel STEM-LAMP assay utilizing stem primers in conjunction with loop-generating and displacement primers.
  • Applied the STEM-LAMP method for the detection of specific pathogens and viruses.
  • Compared the performance of STEM-LAMP with traditional LAMP.

Main Results:

  • STEM-LAMP demonstrated significant improvements in assay speed and sensitivity compared to conventional LAMP.
  • The use of stem primers provided greater flexibility in primer design, including forward/reverse orientations and multiplexing capabilities.
  • STEM-LAMP allowed for optional omission of displacement primers, enhancing assay adaptability.

Conclusions:

  • Stem primers offer a valuable alternative to loop primers in LAMP assays.
  • STEM-LAMP provides enhanced assay speed, sensitivity, and reproducibility for in vitro diagnostic (IVD) assay development.
  • This approach expands primer design options and improves the overall performance of isothermal amplification techniques.