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Related Experiment Videos

Hepadnavirus enhancer and its binding proteins.

S Murakami1, M Uchijima, A Shimoda

  • 1Biophysics Department, Cancer Research Institute, Kanazawa University School of Medicine, Japan.

Gastroenterologia Japonica
|September 1, 1990
PubMed
Summary
This summary is machine-generated.

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A conserved DNA sequence in hepadnavirus enhancers acts as a tumor promoter-inducible enhancer. This sequence binds ubiquitous DNA-binding proteins, distinct from c-jun/fos, and is not affected by hepadnavirus X protein.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Mammalian hepadnavirus genomes contain a conserved 33 bp DNA sequence within the enhancer region.
  • The function of this conserved sequence in enhancer activity and DNA-protein complex formation is not fully understood.

Purpose of the Study:

  • To elucidate the role of the conserved 33 bp DNA sequence in the hepadnavirus enhancer.
  • To investigate the enhancer activity and DNA-protein complex formation capabilities of synthetic DNA sequences related to the conserved region.
  • To determine the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on enhancer activity and DNA-protein complex formation.

Main Methods:

  • Synthesis and testing of various DNA sequences, including tandem copies of 46 bp and 23 bp fragments, for enhancer activity.

Related Experiment Videos

  • Analysis of DNA-protein complex formation using cell extracts (HepG2, HeLa) treated with TPA.
  • Comparison of DNA-protein complexes with those formed by TPA-responsive elements (TREs).

    • Main Results:

    • Two tandem copies of a 46 bp DNA covering the conserved sequence, and two tandem copies of a 23 bp fragment within the sequence, demonstrated enhancer activity.
    • TPA treatment augmented the enhancer activity.
    • TPA stimulation increased DNA-protein complex formation with the 23 bp DNA in HepG2 and HeLa cell extracts.
    • DNA-protein complexes with the 23 bp DNA were found to be similar to, yet distinct from, complexes with TRE DNA.

    Conclusions:

    • The conserved hepadnavirus enhancer sequence is a TPA-inducible enhancer.
    • This enhancer is transactivated by ubiquitous DNA-binding proteins.
    • Hepadnavirus X protein does not appear to directly or indirectly influence DNA-protein complex formation with this conserved sequence.