Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing the evaporation rate of 3D solar evaporators by coating their surface with N-doped graphene and MnCoGe alloy compounds.

Journal of environmental management·2025
Same author

High removal of PS and PET microplastics from tap water by using Fe<sub>2</sub>O<sub>3</sub> porous microparticles and photothermal irradiation with NIR light.

Chemosphere·2024
Same author

Effect of NiAl alloy microparticles deposited in flexible SERS substrates on the limit of detection of rhodamine B molecules.

Nanoscale·2024
Same author

Extensive morphological and behavioural diversity among fourteen new and seven described species in <i>Phytophthora</i> Clade 10 and its evolutionary implications.

Persoonia·2024
Same author

A solar evaporator fabricated from corncob waste for the desalination of seawater and removal of oil/herbicides from contaminated water.

Chemosphere·2023
Same author

Using NIR irradiation and magnetic bismuth ferrite microparticles to accelerate the removal of polystyrene microparticles from the drinking water.

Journal of environmental management·2023

Related Experiment Video

Updated: May 25, 2026

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
07:27

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

Published on: October 21, 2017

Changes in IL12A methylation pattern in livers from mice fed DDC.

J Oliva1, S W French

  • 1Department of Hematology, LaBIOMED, Torrance, 90502, CA 90502, USA. joliva@labiomed.org

Experimental and Molecular Pathology
|January 26, 2012
PubMed
Summary

Mallory-Denk body formation in hepatitis is linked to the Toll-like receptor (TLR) pathway. SAMe supplementation prevented TLR gene upregulation and MDB formation by modulating IL12A gene methylation.

More Related Videos

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
08:44

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice

Published on: April 19, 2020

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
10:17

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

Related Experiment Videos

Last Updated: May 25, 2026

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
07:27

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

Published on: October 21, 2017

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
08:44

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice

Published on: April 19, 2020

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
10:17

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

Area of Science:

  • Hepatology
  • Molecular Biology
  • Immunology

Background:

  • Mallory-Denk body (MDB) formation is a hallmark of alcoholic and non-alcoholic hepatitis.
  • Toll-like receptor (TLR) pathway proteins are implicated in MDB formation.
  • SAMe supplementation has shown potential in preventing MDB formation by counteracting TLR gene upregulation.

Purpose of the Study:

  • To investigate the role of DNA methylation in the TLR pathway genes during MDB formation.
  • To determine how SAMe supplementation affects the methylation patterns of TLR pathway genes, specifically IL12A.
  • To elucidate the mechanism linking IL12A gene expression, methylation, and MDB formation.

Main Methods:

  • Liver DNA was extracted from mice subjected to different feeding conditions (control, DDC, DDC refeeding, DDC+SAMe).
  • A PCR array was employed to analyze the methylation patterns of genes within the mouse TLR pathway.
  • Quantitative analysis focused on the IL12A gene and its promoter methylation status.

Main Results:

  • Twenty-four TLR pathway genes, including IL12A, showed methylation-dependent expression regulation.
  • DDC feeding for 10 weeks and subsequent refeeding reduced IL12A gene methylation.
  • SAMe supplementation partially restored IL12A gene methylation, decreasing promoter methylation compared to DDC groups.

Conclusions:

  • Reduced IL12A gene methylation and subsequent low expression, influenced by DDC, may contribute to MDB formation via IFNg induction.
  • SAMe supplementation modulates IL12A methylation, suggesting a potential therapeutic mechanism in hepatitis.
  • Understanding the epigenetic regulation of IL12A offers insights into MDB pathogenesis.