Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries.

Nature communications·2026
Same author

Tackling matrix effects in biosensor-based analysis of untreated blood plasma.

Analytical and bioanalytical chemistry·2026
Same author

A novel microfluidic multichannel electrochemical cell for multiplexed monitoring of water pollutants.

Lab on a chip·2025
Same author

Front-illuminated surface plasmon resonance biosensor for the study of light-responsive proteins and their interactions.

Biosensors & bioelectronics·2025
Same author

Large gold nanoparticle release assay for attomolar detection of miRNA related to myelodysplastic neoplasms.

Talanta·2025
Same author

Surface plasmon resonance biosensor for environmental detection of tramadol.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: May 25, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

High-resolution biosensor based on localized surface plasmons.

Marek Piliarik1, Hana Sípová, Pavel Kvasnička

  • 1Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.

Optics Express
|January 26, 2012
PubMed
Summary

A novel biosensor utilizing localized surface plasmons (LSP) offers high sensitivity for DNA detection. This gold nanorod array biosensor matches conventional surface plasmon resonance (SPR) performance with lower molecule density requirements.

More Related Videos

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

Related Experiment Videos

Last Updated: May 25, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Biosensors are crucial for detecting biological molecules.
  • Conventional Surface Plasmon Resonance (SPR) biosensors offer high sensitivity but require significant molecule densities.
  • Localized Surface Plasmon (LSP) biosensing presents a potential alternative for enhanced detection.

Purpose of the Study:

  • To develop and characterize a new biosensor based on localized surface plasmons (LSP) using gold nanorods.
  • To evaluate the sensitivity and performance of the LSP biosensor for DNA hybridization detection.
  • To compare the LSP biosensor's performance against a conventional high-resolution SPR biosensor.

Main Methods:

  • Fabrication of a biosensor utilizing an array of gold nanorods.
  • Implementation of total internal reflection imaging in polarization contrast.
  • Characterization of biosensor sensitivity and detection of DNA hybridization.
  • Comparative analysis with a reference SPR biosensor experiment.

Main Results:

  • The LSP-based biosensor demonstrated high sensitivity in detecting DNA hybridization.
  • Performance was comparable to a conventional SPR biosensor.
  • The LSP biosensor required significantly lower surface densities of interacting molecules.
  • Achieved a limit of detection of 100 pM and a surface density resolution of 35 fg×mm-2.

Conclusions:

  • The developed LSP biosensor provides a sensitive and efficient platform for molecular detection.
  • This technology enables high-performance biosensing with reduced sample requirements.
  • The LSP biosensor shows promise for various applications in molecular diagnostics and analysis.