Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One-year follow-up after fractionated ultra-high-dose-rate FLASH radiotherapy in patient with extramammary Paget disease of the scrotum.

Precision radiation oncology·2026
Same author

Pilot longitudinal integrated transcriptomic-metabolomic study reveals immune and metabolic signatures in non-hospitalized healthcare workers with long COVID.

Frontiers in cellular and infection microbiology·2026
Same author

Inheritance patterns of mitochondrial DNA in multi-generational maternal pedigrees: Insights from mitochondrial whole-genome sequencing.

Forensic science international. Genetics·2026
Same author

Editorial: Managing COVID-19 in heart and lung transplantation: clinical challenges and emerging solutions.

Frontiers in surgery·2026
Same author

Infrapatellar Fat Pad Preservation versus Resection in Total Knee Arthroplasty.

NEJM evidence·2026
Same author

Donor-derived cell-free DNA associated with increased risk of chronic lung allograft dysfunction and mortality: Implications of immunosuppression and retransplantation.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026

Related Experiment Video

Updated: May 25, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
10:51

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Published on: April 16, 2014

Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy.

Qiang Wu1, Shangyu Guo, Yinxing Ma

  • 1The MOE Key Laboratory of Weak Light Nonlinear Photonics, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin, China.

Optics Express
|January 26, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel 3D fluorescence lifetime microscopy technique. The method allows for non-invasive dynamic measurements in physics, chemistry, and biology without disturbing the sample.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Related Experiment Videos

Last Updated: May 25, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
10:51

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Published on: April 16, 2014

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Chemical Physics

Background:

  • Traditional fluorescence microscopy can disturb delicate samples during 3D imaging.
  • Dynamic processes in physics, chemistry, and biology often require non-invasive observation methods.

Purpose of the Study:

  • To develop a three-dimensional fluorescence lifetime microscopy technique.
  • To enable non-invasive, dynamic measurements in various scientific fields.
  • To achieve high-resolution 3D imaging without sample manipulation.

Main Methods:

  • Combines wide-field fluorescence lifetime imaging with remote optical refocusing.
  • Utilizes a fluorescent microsphere for system performance testing.
  • Enables time-resolved fluorescence spectroscopy.

Main Results:

  • Successfully demonstrated three-dimensional fluorescence lifetime microscopy.
  • Validated the system's performance for non-invasive 3D measurements.
  • Achieved successful time-resolved fluorescence spectroscopy.

Conclusions:

  • The developed system provides a powerful tool for undisturbed 3D dynamic measurements.
  • This technique advances non-invasive imaging in biological, chemical, and physical sciences.
  • The method opens new possibilities for studying dynamic phenomena in real-time.