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Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Neogenin regulates Sonic Hedgehog pathway activity during digit patterning.

Mingi Hong1, Karen A Schachter, Guoying Jiang

  • 1Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|January 26, 2012
PubMed
Summary
This summary is machine-generated.

Neogenin (Neo1) regulates Sonic Hedgehog (SHH) signaling for proper digit development. Neo1 mutations cause polydactyly and alter SHH target gene expression, impacting limb patterning.

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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Digit patterning involves Sonic Hedgehog (SHH), FGF, and BMP signaling pathways.
  • GLI3 is a key regulator of digit number and identity within the SHH pathway.
  • Neogenin (Neo1) is a cell surface protein involved in BMP signaling, but its role in SHH signaling and digit patterning is unknown.

Purpose of the Study:

  • To investigate the role of neogenin (Neo1) in digit patterning.
  • To determine if Neo1 interacts with SHH or BMP signaling pathways during limb development.

Main Methods:

  • Analysis of Neo1 mutant mice, including those with combined Neo1 and Gli3 mutations.
  • Examination of SHH and BMP target gene expression in limb buds.
  • In vitro studies using embryo fibroblasts from Neo1 mutant mice.

Main Results:

  • Neo1 mutant mice exhibit preaxial polydactyly with low penetrance.
  • SHH, but not BMP, target gene expression is altered in Neo1 mutant limb buds.
  • Neogenin constrains digit number but is also required for severe polydactyly in Gli3 mutants. Neo1 mutant fibroblasts show enhanced SHH pathway activation.

Conclusions:

  • Neogenin plays a crucial role in regulating SHH signaling within the limb bud.
  • Neogenin is essential for proper digit patterning by modulating SHH pathway activity.